Publications

Cherrak Y, Filella-Merce I, Schmidt V, Byrne D, Sgoluppi V, Chaiaheloudjou R, Betzi S, Morelli X, Nilges M, Pellarin R, Durand E. Inhibiting Type VI Secretion System Activity with a Biomimetic Peptide Designed To Target the Baseplate Wedge Complex. mBio. 2021 Aug 31;12(4):e0134821. doi: 10.1128/mBio.01348-21. Epub 2021 Aug 10. PMID: 34372705 .

Flaugnatti N, Rapisarda C, Rey M, Beauvois SG, Nguyen VA, Canaan S, Durand E, Chamot‐Rooke J, Cascales E, Fronzes R & Journet L. Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike. EMBO Journal (2020), Volume 39, Issue 11 https://doi.org/10.15252/embj.2019104129

Crepin A, Kučerová Z, Kosta A, Durand E, Caffarri S. Isolation and characterization of a large Photosystem I-Light Harvesting complex II supercomplex with an additional Lhca1-a4 dimer in Arabidopsis. Plant J. 2019 Dec 6. doi: 10.1111/tpj.14634. [Epub ahead of print] PMID: 31811681

Cherrak Y, Flaugnatti N, Durand E, Journet L, Cascales E. Structure and Activity of the Type VI Secretion System. Microbiol Spectr. 2019 Jul;7(4). doi: 10.1128/microbiolspec.PSIB-0031-2019. Review. PMID: 31298206

Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, Eric Cascales, Martin Pilhofer, Eric Durand*, Remi Fronzes* (co-corresponding author*). In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex. EMBO Journal. 2019 Mar 15. pii: e100886. doi: 10.15252/embj.2018100886.  PMID: 30877094

Cherrak Y, Rapisarda C, Pellarin R, Bouvier G, Bardiaux B, Allain F, Malosse C, Rey M, Chamot-Rooke J, Cascales E, Fronzes R, Durand E. Biogenesis and structure of a type VI secretion baseplate. Nat Microbiol. 2018 Dec;3(12):1404-1416. doi: 10.1038/s41564-018-0260-1. Epub 2018 Oct 15. PMID: 30323254

Zoued A, Duneau JP, Durand E, España AP, Journet L, Guerlesquin F, Cascales E. Tryptophan-mediated Dimerization of the TssL Transmembrane Anchor Is Required for Type VI Secretion System Activity. J Mol Biol. 2018 Mar 30;430(7):987-1003. doi: 10.1016/j.jmb.2018.02.008. Epub 2018 Feb 17. PMID: 29458124

Nguyen VS, Douzi B, Durand E, Roussel A, Cascales E, Cambillau C. Towards a complete structural deciphering of Type VI secretion system. Curr Opin Struct Biol. 2018 Apr;49:77-84. doi: 10.1016/j.sbi.2018.01.007. Epub 2018 Feb 2. PMID: 29414515

Douzi B, Trinh NTT, Michel-Souzy S, Desmyter A, Ball G, Barbier P, Kosta A, Durand E, Forest KT, Cambillau C, Roussel A, Voulhoux R. Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics. mBio. 2017 Oct 17;8(5):e01185-17. doi: 10.1128/mBio.01185-17. PMID: 29042493

Zoued A, Durand E, Santin YG, Journet L, Roussel A, Cambillau C, Cascales E. TssA: The cap protein of the Type VI secretion system tail. Bioessays. 2017 Oct;39(10). doi: 10.1002/bies.201600262. Epub 2017 Aug 17. PMID: 28817192

Durand E, Lloubes R. Large Complexes: Cloning Strategy, Production, and Purification. Methods Mol Biol. 2017;1615:299-309. doi: 10.1007/978-1-4939-7033-9_24.

Nguyen VS, Logger L, Spinelli S, Legrand P, Huyen Pham TT, Nhung Trinh TT, Cherrak Y, Zoued A, Desmyter A, Durand E, Roussel A, Kellenberger C, Cascales E, Cambillau C. Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nature Microbiol. 2017 Jun 26;2:17103. doi: 10.1038/nmicrobiol.2017.103.

Logger L, Aschtgen MS, Guérin M, Cascales E, Durand E*. (corresponding author*) Molecular dissection of the interface between the Type VI secretion TssM cytoplasmic domain and the TssG baseplate component. Journal of Molecular Biology. 2016 Nov 6;428(22):4424-4437.

Zoued A, Cassaro CJ, Durand E, Douzi B, España AP, Cambillau C, Journet L, Cascales E. Structure-function analysis of the TssL cytoplasmic domain reveals a new interaction between the Type VI secretion baseplate and membrane complexes. Journal of Molecular Biology. 2016 Nov 6;428(22):4413-4423.

Vincent M, Durand E, Cascales E. The PorX Response Regulator of the Porphyromonas gingivalis PorXY Two-Component System Does Not Directly Regulate the Type IX Secretion Genes but Binds the PorL Subunit. Front Cell Infect Microbiol. 2016 Aug 31;6:96.

Sana TG, Flaugnatti F, Lugo KA, Lam LH, Jacobson A, Baylot V, Durand E, Journet L, Cascales E, and Monack DM. Salmonella typhimurium utilizes a T6SS-mediated antibacterial weapon to establish in the host gut. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5044-51.

Zoued A1, Durand E1*, Brunet YR, Spinelli S, Douzi B, Guzzo M, Flaugnatti N, Legrand P, Journet L, Fronzes R, Mignot T, Cambillau C, Cascales E*. (co-first author1 & co-corresponding author*) Priming and polymerization of a bacterial contractile tail structure. Nature. 2016 Mar 3;531(7592):59-63.

Durand E, Nguyen VS, Zoued A, Logger L, Péhau-Arnaudet G, Aschtgen M-S, Spinelli S, Desmyter A Bardiaux B, Dujeancourt A, Roussel A, Cambillau C, Cascales E and Fronzes R. Biogenesis and structure of a bacterial Type VI secretion membrane core complex. Nature. 2015 Jul 30;523(7562):555-60

Nguyen VS, Logger L, Spinelli S, Desmyter A, Le TT, Kellenberger C, Douzi B, Durand E, Roussel A, Cascales E, Cambillau C. Inhibition of type VI secretion by an anti-TssM llama nanobody. PLoS One. 2015 Mar 26;10(3):e0122187

Durand E, Cambillau C, Cascales E, Journet L. VgrG, Tae, Tle, and beyond: the versatile arsenal of Type VI secretion effectors. Trends Microbiol. 2014 Sep;22(9):498-507.

Zoued A, Brunet YR, Durand E, Aschtgen MS, Logger L, Douzi B, Journet L, Cambillau C, Cascales E. Architecture and assembly of the Type VI secretion system. Biochim Biophys Acta. 2014 Aug;1843(8):1664-73.

Douzi B, Spinelli S, Blangy S, Roussel A, Durand E, Brunet YR, Cascales E, Cambillau C. Crystal structure and self-interaction of the type VI secretion tail-tube protein from enteroaggregative Escherichia coli. PLoS One. 2014 Feb 14;9(2):e86918

Blocquel D & Habchi J, Durand E, Sevajol M, Ferron F, Erales J, Papageorgiou N, Longhi S. Coiled-coil deformations in crystal structures: the measles virus phosphoprotein multimerization domain as an illustrative example. Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1589-603.

Zoued A, Durand E, Bebeacua C, Brunet YR, Douzi B, Cambillau C, Cascales E, and Journet L. TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. Journal of Biological Chemistry published 2013 Sep 20;288(38):27031-41

Gueguen E, Durand E, Zhang X-Y, d’Amalric Q, Journet L, Cascales E. Expression of a Yersinia pseudotuberculosis Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator. PLOS ONE, 2013 Jun 19;8(6):e66615.

Durand E, Derrez E, Audoly G, Spinelli S, Ortiz-Lombardia M, Raoult D, Cascales E, Cambillau C. Crystal structure of the VgrG1 actin cross-linking domain of the Vibrio cholerae Type VI secretion system. Journal of Biological Chemistry 2012 Nov 2;287(45):38190-9.

Durand E, Aschtgen M-S, Zoued A, Spinelli S, Watson PJH, Cambillau C and Cascales E. Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. Journal of Biological Chemistry 2012 Apr 20;287(17):14157-68. Epub 2012 Feb 27.

Felisberto-Rodrigues C, Durand E, Aschtgen M-S, Blangy S, Ortiz-Lombardia M, Douzi B, Cambillau C and Cascales E. Towards a Structural Comprehension of Bacterial Type VI Secretion Systems: Characterization of the TssJ-TssM Complex of an Escherichia coli Pathovar. PLoS Pathog. 2011 Nov;7(11):e1002386. Epub 2011 Nov 10.

Durand E, Alphonse S, Brochier-Armanet C, Ball G, Douzi B, Filloux A, Bernard C, Voulhoux R. The assembly mode of the pseudopilus: a hallmark to distinguish a novel secretion system subtype. Journal of Biological Chemistry 2011 Jul 8;286(27):24407-16. Epub 2011 May 17.

Franz LP, Douzi B, Durand E, Dyer DH, Voulhoux R, Forest KT. Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system. Acta Crystallogr D Biol Crystallogr. 2011 Feb;67(Pt 2):124-30. Epub 2011 Jan 15.

Alphonse S, Durand E, Douzi B, Waegele B, Darbon H, Filloux A, Voulhoux R and Bernard C. 2009. Structure of the Pseudomonas aeruginosa XcpT pseudopilin, a major component of the Type II secretion system. Journal of Structural Biology. Jan;169(1):75-80.

Douzi B, Durand E, Bernard C, Alphonse S, Cambillau C, Filloux A, Tegoni M and Voulhoux R. 2009. The XcpV/GspI pseudopilin has a central role in the assembly of a quaternary complex within the T2SS pseudopilus. Journal of Biological Chemistry. Dec 11; 284(50): 34580-9.

Durand E, Waksman G, Receveur-Brechot V. Structural insights into the membrane-extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system. BMC Struct Biol. 2011 Jan 25;11:4.

Durand E, Oomen C and Waksman G. 2010. Biochemical dissection of the ATPase TraB, the VirB4 homologue of the E. coli pKM101 conjugation machinery. Journal of Bacteriology, May 2010, Vol 192, No 9.

Durand E, Verger D, Toste Rego A, Chandran V, Meng G, Fronzes R & Waksman G. 2009. Structural biology of bacterial secretion systems in Gram-negative pathogens - Potential for new drug targets. Infectious Disorders – Drug Targets. Nov 9(5): 518-47. Review.

Arts J, de Groot A, Ball G, Durand E, El Khattabi M, Filloux A, Tommassen J, Koster M. 2007. Interaction domains in the Pseudomonas aeruginosa type II secretory apparatus component XcpS (GspF). Microbiology. May;153(Pt 5):1582-92.

Michel GP, Durand E, Filloux A. 2007. XphA/XqhA, a novel GspCD subunit for type II secretion in Pseudomonas aeruginosa. J Bacteriol. May;189(10):3776-83. Epub 2007 Mar 9.

Durand E, Michel G, Voulhoux R, Kürner J, Bernadac A, and Filloux A. 2005. XcpX controls biogenesis of the Pseudomonas aeruginosa XcpT-containing pseudopilus. Journal of Biological Chemistry 280 (36): 31378-31389.

Durand E, Bernadac A, Ball G, Lazdunski A, Sturgis JN, and Filloux A. 2003. Type II protein secretion in Pseudomonas aeruginosa: the pseudopilus is a multifibrillar and adhesive structure. Journal of Bacteriology 185:2749-2758.

Ball G., Durand E, Lazdunski A, and Filloux A. 2002. A novel type II secretion system in Pseudomonas aeruginosa. Mol. Microbiol. 43:475-485.

Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, Eric Cascales, Martin Pilhofer, Eric Durand*, Remi Fronzes* (co-corresponding author*). In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex. EMBO Journal. 2019 Mar 15. pii: e100886. doi: 10.15252/embj.2018100886. PMID: 30877094

Cherrak Y, Rapisard C, Pellarin R, Bouvier G, Bardiaux B, Allain F, Malosse C, Rey M, Chamot-Rooke J, Cascales E, Fronzes R and Durand E* (corresponding author*). Biogenesis and structure of a type VI secretion baseplate. Nature Microbiol. 2018 Dec;3(12):1404-1416. doi: 10.1038/s41564-018-0260-1

Zoued A, Duneau JP, Durand E, España AP, Journet L, Guerlesquin F, Cascales E. Tryptophan-mediated dimerization of the TssL transmembrane anchor is required for Type VI secretion system activity. Journal of Molecular Biology. 2018 Feb 16. pii: S0022-2836(18)30079-2. doi: 10.1016/j.jmb.2018.02.008.

Nguyen VS, Douzi B, Durand E, Roussel A, Cascales E, Cambillau C. Towards a complete structural deciphering of Type VI secretion system. Curr Opin Struct Biol. 2018 Apr;49:77-84. doi: 10.1016/j.sbi.2018.01.007. Epub 2018 Feb 2. Review. PMID: 29414515

Douzi B, Trinh NTT, Michel-Souzy S, Desmyter A, Ball G, Barbier P, Kosta A, Durand E, Forest KT, Cambillau C, Roussel A, Voulhoux R. Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics. MBio. 2017 Oct 17;8(5). pii: e01185-17.

Zoued A, Durand E, Santin YG, Journet L, Roussel A, Cambillau C, Cascales E. TssA: The cap protein of the Type VI secretion system tail. Bioessays. 2017 Aug 17. doi: 10.1002/bies.201600262. Review